KMID : 0882420040670020131
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Korean Journal of Medicine 2004 Volume.67 No. 2 p.131 ~ p.145
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Detection of Helicobacter pylori in oral cavity by polymerase chain reaction
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Yang Chun-Ho
Kim Kang-Ju
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Abstract
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Background: Many authors hypothesized that Helicobacter pylori (H. pylori), a causative agent of gastric disease, might act as a potential antigen in salivary glands. H. pylori has been suggested to be transmitted via oral route.
Methods: To investigate the transmission of H. pylori via oral route, saliva samples were taken from the patients with gastric disease. After total £ÄNA was isolated from each saliva sample, amplification of H. pylori urease gene fragment was performed by polymerase chain reaction (PCR) with the isolated DNA as template. PCR-amplified DNA fragments were obtained from seven of 42 patients, the amplified DNA fragments were sequenced by automatic DNA sequencer. Biopsy materials from 2 patients of CLO test-positive with gastric disease were examined and photographed using a light and an electron microscope.
Results: The nucleotide sequence of PCR products showed high homology with H. pylori 26695 urease (98%), and the deduced amino acid sequence was identical to the urease ¥ásubunit of H. pylori J99. The cytosine in the 272th position of urease A nucleotide sequence showed the tendency of high prevalence in the patient with healthy periodontium. Electron micrograph showed helical cell profile of H. pylori on surface epithelial lining cells.
Conclusion: These results suggest that there might be a transmission of H. pylori via oral route.(Korean J Med 67:131-145, 2004)
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KEYWORD
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Helicobacter pylori, PCR
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